Protein Ladder Immunofluorescence

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.

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Found 6 matching solutions for this experiment

Biotinylated Protein Ladder Detection Pack #7727

Cell Signaling Technology

Upstream tips	Protocol tips	Downstream tips
• The molecular weight ladder is a mixture of purified proteins covalently coupled to biotin that resolve to 10 bands that have a size range of 9-200 kDa.	• Heat the ladder to 95-100ºC for 2 minutes. • To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells. • Perform Chemiluminescent detection according to manufacturer's instructions except, at the stage of secondary antibody incubation with the blot. • Concurrently add the anti-biotin HRP-linked antibody (provided) at a dilution of 1:1000.

Upstream tips
• The molecular weight ladder is a mixture of purified proteins covalently coupled to biotin that resolve to 10 bands that have a size range of 9-200 kDa.
Protocol tips
• Heat the ladder to 95-100ºC for 2 minutes. • To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells. • Perform Chemiluminescent detection according to manufacturer's instructions except, at the stage of secondary antibody incubation with the blot. • Concurrently add the anti-biotin HRP-linked antibody (provided) at a dilution of 1:1000.

Tau Protein Ladder, 6 isoforms human

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
• Contains 6 recombinant Tau proteins expressed in E. coli, which comprise six Tau isoforms with molecular weights of 45,900, 42,600, 42,900, 39,700, 40,000, and 36,800 respectively. • No histidine-tags are present in the proteins.	• For Immunoelectrophoretic load: 10-20 μL using recombinant Tau protein marker • For Western Blot load: 2-5 μL using recombinant Tau protein marker

Upstream tips
• Contains 6 recombinant Tau proteins expressed in E. coli, which comprise six Tau isoforms with molecular weights of 45,900, 42,600, 42,900, 39,700, 40,000, and 36,800 respectively. • No histidine-tags are present in the proteins.
Protocol tips
• For Immunoelectrophoretic load: 10-20 μL using recombinant Tau protein marker • For Western Blot load: 2-5 μL using recombinant Tau protein marker

1 Relevant paper

WesternSure® Pre-stained Chemiluminescent Protein Ladder

LI-COR

Upstream tips	Protocol tips	Downstream tips
• Contains nine recombinant protein bands (8 to 260 kDa)	Western blot workflows with chemiluminescent protein ladders are prone to complications: • Your ladder may require strep-tagged HRP • Your ladder may rely on IgG binding sites to be detected by an HRP secondary antibody • Trial and error may be required to optimize loading volume for your ladder

Upstream tips
• Contains nine recombinant protein bands (8 to 260 kDa)
Protocol tips
Western blot workflows with chemiluminescent protein ladders are prone to complications: • Your ladder may require strep-tagged HRP • Your ladder may rely on IgG binding sites to be detected by an HRP secondary antibody • Trial and error may be required to optimize loading volume for your ladder

Western Blotting Protein Standards

Bio-Rad Laboratories

Upstream tips	Protocol tips	Downstream tips
• Protein standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD.		• Fluorescent detection — blue bands can be visualized with a 635 nm laser or red LED. • Pink bands can be visualized with a 532 nm laser or green LED.

Upstream tips
• Protein standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD.
Downstream tips
• Fluorescent detection — blue bands can be visualized with a 635 nm laser or red LED. • Pink bands can be visualized with a 532 nm laser or green LED.

Chameleon® Vue Pre-stained Protein Ladder

LI-COR

Upstream tips	Protocol tips	Downstream tips
• Contains nine recombinant protein bands (8 – 260 kDa)	• This pre-stained protein ladder can be used for chemiluminescent detection when paired with the WesternSure® Pen. • It is optimized for use with Bis-Tris and Tris-Glycine gels.

Upstream tips
• Contains nine recombinant protein bands (8 – 260 kDa)
Protocol tips
• This pre-stained protein ladder can be used for chemiluminescent detection when paired with the WesternSure® Pen. • It is optimized for use with Bis-Tris and Tris-Glycine gels.

1 video

Odyssey® One-Color Protein Molecular Weight Marker

LI-COR

Upstream tips	Protocol tips	Downstream tips
• Includes ten protein bands from 10 to 250 kDa.		• Fluorescent detection — blue bands can be visualized with a 635 nm laser or red LED. • Pink bands can be visualized with a 532 nm laser or green LED.

Upstream tips
• Includes ten protein bands from 10 to 250 kDa.
Downstream tips
• Fluorescent detection — blue bands can be visualized with a 635 nm laser or red LED. • Pink bands can be visualized with a 532 nm laser or green LED.

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