Reporter gene assay β-galactosidase substrates - CHO

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 2 matching solutions for this experiment

Luminescent β-galactosidase Detection Kit II

Takara Bio Inc

Upstream tips	Protocol tips	Downstream tips
Warm enough Reaction Buffer and Reaction Substrate for the entire experiment to room temperature	Add 200 µl of the Reaction Buffer Mixture to each cell lysate and mix gently. Incubate at room temperature (20–25°C) for 60 min

Upstream tips
Warm enough Reaction Buffer and Reaction Substrate for the entire experiment to room temperature
Protocol tips
Add 200 µl of the Reaction Buffer Mixture to each cell lysate and mix gently. Incubate at room temperature (20–25°C) for 60 min

Manufacturer protocol Publication protocol 1 Relevant paper

Senescence Cells Histochemical Staining Kit

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
Seed 1×10^5 and treat with C-1305	Fix with with 2% formaldehyde and 0.2% glutaraldehyde in PBS for 5 min. Add 1 ml of the Staining Mixture per well and incubate at 37 °C without CO2 until the cells are stained blue

Upstream tips
Seed 1×10^5 and treat with C-1305
Protocol tips
Fix with with 2% formaldehyde and 0.2% glutaraldehyde in PBS for 5 min. Add 1 ml of the Staining Mixture per well and incubate at 37 °C without CO2 until the cells are stained blue

Manufacturer protocol Publication protocol 1 Relevant paper

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