Reporter gene assay β-galactosidase substrates - HepG2

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 4 matching solutions for this experiment

Senescence Cells Histochemical Staining Kit

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	Fix cells in 2% paraformaldehyde and 0.02% glutaraldehyde in PBS Add 1 ml of the Staining Mixture per well and incubate for 16 h at 37 °C without CO2 until the cells are stained blue

Protocol tips
Fix cells in 2% paraformaldehyde and 0.02% glutaraldehyde in PBS Add 1 ml of the Staining Mixture per well and incubate for 16 h at 37 °C without CO2 until the cells are stained blue

Manufacturer protocol Publication protocol 1 Relevant paper

β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

Promega

Upstream tips	Protocol tips	Downstream tips
	Add 150µl of Assay 2X Buffer to sample. Incubate the reactions at 37°C for 24 hours	Read the absorbance at 420nm

Protocol tips
Add 150µl of Assay 2X Buffer to sample. Incubate the reactions at 37°C for 24 hours
Downstream tips
Read the absorbance at 420nm

Manufacturer protocol Publication protocol 1 Relevant paper

Senescence β-Galactosidase Staining Kit - Cell Signaling

Cell Signaling Technology

Upstream tips	Protocol tips	Downstream tips
	Fix cells with with 2% formaldehyde/0.2% glutaraldehyde. Incubate for 12 hours at 37 ° C without Co2

Protocol tips
Fix cells with with 2% formaldehyde/0.2% glutaraldehyde. Incubate for 12 hours at 37 ° C without Co2

Manufacturer protocol Publication protocol 1 Relevant paper

Senescence β-Galactosidase Staining Kit - Beyotime

Beyotime

Upstream tips	Protocol tips	Downstream tips
	Add 1 ml of the β-Galactosidase Staining Solution Incubate overnight at 37 ° C without Co2

Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution Incubate overnight at 37 ° C without Co2

Manufacturer protocol Publication protocol 1 Relevant paper

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