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Found 3 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
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- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer). |
- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
|
Protocol tips |
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer). |
Downstream tips |
- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
|
Upstream tips |
Protocol tips |
Downstream tips |
- To decrease RNase activity,process starting material immeditely or store at 80C.
- To increase nucleic yield or purity store all buffer at +15C to +25C. |
- To decrease RNase activity,use eluted RNA directly in downstream procedure or store at -80C immediately. |
|
Upstream tips |
- To decrease RNase activity,process starting material immeditely or store at 80C.
- To increase nucleic yield or purity store all buffer at +15C to +25C. |
Protocol tips |
- To decrease RNase activity,use eluted RNA directly in downstream procedure or store at -80C immediately. |
Upstream tips |
Protocol tips |
Downstream tips |
|
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.
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Protocol tips |
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.
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