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Found 3 matching solutions for this experiment
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Whole lung tissue was homogenized prior to RNA extraction (BeadBlaster 24, Benchmark Scientific) in the presence of lysis buffer (RLT Plus, Qiagen). Total RNA from whole lung tissue and sorted alveolar macrophages and alveolar type II cells was extracted (AllPrep DNA/RNA Mini Kit, Qiagen) and quality and quantity were assessed (TapeStation 4200, Agilent). |
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Protocol tips |
Whole lung tissue was homogenized prior to RNA extraction (BeadBlaster 24, Benchmark Scientific) in the presence of lysis buffer (RLT Plus, Qiagen). Total RNA from whole lung tissue and sorted alveolar macrophages and alveolar type II cells was extracted (AllPrep DNA/RNA Mini Kit, Qiagen) and quality and quantity were assessed (TapeStation 4200, Agilent). |
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For the cell lines, RNA was extracted from the cells using the RNEasy® kit (Qiagen), generating high‐quality total RNA (i.e., RIN > 8) that was used as input material for library construction with Illumina TruSeq Stranded mRNA reagents. |
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For the cell lines, RNA was extracted from the cells using the RNEasy® kit (Qiagen), generating high‐quality total RNA (i.e., RIN > 8) that was used as input material for library construction with Illumina TruSeq Stranded mRNA reagents. |
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