RNA isolation / purification Tissue - Human Saliva

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4 years ago

4 years ago by Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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4 years ago

4 years ago by Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Found 1 matching solution for this experiment

RNeasy Protect Saliva Mini Kit (50)

Qiagen

Protocol tips
For stabilization, use approximately 10 µl of saliva
Follow manufacturer’s instructions
Manufacturer protocol Publication protocol 1 Relevant paper
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