No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 3 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
|
- For RNA to dissolve better and to yeild high levels, preheat elution buffer at 95C.
- Addition of β-mercaptoethanol in lysis buffer can be skipped because the phenol will do the job of nuclease inactivation
- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step
|
- Do vortex for 2-plus minutes during Acid-Phenol:Chloroform extraction step. This will facilitate the removal of tightly bound proteins typically associated with RNA |
Protocol tips |
- For RNA to dissolve better and to yeild high levels, preheat elution buffer at 95C.
- Addition of β-mercaptoethanol in lysis buffer can be skipped because the phenol will do the job of nuclease inactivation
- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step
|
Downstream tips |
- Do vortex for 2-plus minutes during Acid-Phenol:Chloroform extraction step. This will facilitate the removal of tightly bound proteins typically associated with RNA |
Upstream tips |
Protocol tips |
Downstream tips |
- Aliquot rDNase and store at -20 °C.
|
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer. |
|
Upstream tips |
- Aliquot rDNase and store at -20 °C.
|
Protocol tips |
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer. |
Upstream tips |
Protocol tips |
Downstream tips |
- This kit can be used for the isolation of RNA from up to 1x10^6 cells
|
|
|
Upstream tips |
- This kit can be used for the isolation of RNA from up to 1x10^6 cells
|
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!