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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| - 0, 500, 1000, 5000, and 1000 wild type and autobioluminescent HEK293 cells were platted |
- Cells were incubated at 37°C and 5% CO2 for 18 h before the addition of 20 µl 125 µM H2O2 substrate.
- each well was then incubated for an additional 6 h followed by bioluminescent measurements of H2O2 levels. |
|
| Upstream tips |
| - 0, 500, 1000, 5000, and 1000 wild type and autobioluminescent HEK293 cells were platted |
| Protocol tips |
- Cells were incubated at 37°C and 5% CO2 for 18 h before the addition of 20 µl 125 µM H2O2 substrate.
- each well was then incubated for an additional 6 h followed by bioluminescent measurements of H2O2 levels. |
| Upstream tips |
Protocol tips |
Downstream tips |
|
- Cells were treated with 0.33 mm H2O2 |
- ROS levels were measured after 1 and 4 h of treatment. |
| Protocol tips |
| - Cells were treated with 0.33 mm H2O2 |
| Downstream tips |
| - ROS levels were measured after 1 and 4 h of treatment. |
| Upstream tips |
Protocol tips |
Downstream tips |
|
- Cells were treated with superoxide Detection Solution and then incubated for 30 min |
|
| Protocol tips |
| - Cells were treated with superoxide Detection Solution and then incubated for 30 min |
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