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Found 7 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| - Cells should be healthy and not overcrowded since results of the experiments will depend significantly on the cells’ condition. |
- The cells were stained using the two‐color ROS Detection Kit.
-Cells should be pre-treated with the ROS Inhibitor at least 30 minutes prior to induction. |
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| Upstream tips |
| - Cells should be healthy and not overcrowded since results of the experiments will depend significantly on the cells’ condition. |
| Protocol tips |
- The cells were stained using the two‐color ROS Detection Kit.
-Cells should be pre-treated with the ROS Inhibitor at least 30 minutes prior to induction. |
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- 1X DCFH-DA/media solution contains 5% methanol. For cells that are sensitive to methanol,
we recommend instead preparing a 0.1X (100 µM) solution of DCFH-DA in cell culture media. |
- Due to light-induced auto-oxidation, DCFH-DA solutions at any concentration must be
protected from light. |
| Protocol tips |
- 1X DCFH-DA/media solution contains 5% methanol. For cells that are sensitive to methanol,
we recommend instead preparing a 0.1X (100 µM) solution of DCFH-DA in cell culture media. |
| Downstream tips |
- Due to light-induced auto-oxidation, DCFH-DA solutions at any concentration must be
protected from light. |
| Upstream tips |
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- 2 × 10^5 cells/well were seeded.
- Cells were either treated with or without diosgenin. |
- Cells were incubated with 5 μM carboxy-H2DCFDA for 15 min at 37°C.
- Cells were washed with PBS twice, trypsinized, and resuspended in OptiMem I medium. |
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| Upstream tips |
- 2 × 10^5 cells/well were seeded.
- Cells were either treated with or without diosgenin. |
| Protocol tips |
- Cells were incubated with 5 μM carboxy-H2DCFDA for 15 min at 37°C.
- Cells were washed with PBS twice, trypsinized, and resuspended in OptiMem I medium. |
| Upstream tips |
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- Cells were cultured and treated with phorbol myristate acetate (PMA) and ionomycin and incubated for 30 min.
- 5 × 10^5 were used for as ROS assay. |
- 10 µM final concentration of Carboxy-H2DCFDA was used incubated for 30 min. |
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| Upstream tips |
- Cells were cultured and treated with phorbol myristate acetate (PMA) and ionomycin and incubated for 30 min.
- 5 × 10^5 were used for as ROS assay. |
| Protocol tips |
| - 10 µM final concentration of Carboxy-H2DCFDA was used incubated for 30 min. |
| Upstream tips |
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| - 2.5 × 104 cells were seeded for an assay |
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- The fluorescence intensity was measured using a fluorescence plate reader at Ex/Em. = 488/525 nm. |
| Upstream tips |
| - 2.5 × 104 cells were seeded for an assay |
| Downstream tips |
| - The fluorescence intensity was measured using a fluorescence plate reader at Ex/Em. = 488/525 nm. |
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- Due to light-induced auto-oxidation, the stock DCF-DiOxyQ solution and all subsequent
DCF-DiOxy and DCFH solutions must be protected from light. |
| Downstream tips |
- Due to light-induced auto-oxidation, the stock DCF-DiOxyQ solution and all subsequent
DCF-DiOxy and DCFH solutions must be protected from light. |
| Upstream tips |
Protocol tips |
Downstream tips |
- 5 × 10^4 cells were seeded for the assay.
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- As a negative control cells were treated with 15 µg/mL NaB |
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| Upstream tips |
- 5 × 10^4 cells were seeded for the assay.
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| Protocol tips |
| - As a negative control cells were treated with 15 µg/mL NaB |
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