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Found 1 matching solution for this experiment
siGENOME Rat Nrp1 siRNA + DharmaFECT 3 Transfection Reagent
| Upstream tips |
Protocol tips |
Downstream tips |
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Prepare transfection mixture in in serum-free medium and incubate for 20 min at room temperature.
After 4 h transfection medium replace with complete medium.
siRNA concentration- 100 nM
Incubate cells at 37°C in 5% CO2 for 24–48 hours.
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| Protocol tips |
Prepare transfection mixture in in serum-free medium and incubate for 20 min at room temperature.
After 4 h transfection medium replace with complete medium.
siRNA concentration- 100 nM
Incubate cells at 37°C in 5% CO2 for 24–48 hours.
|
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