Stem cell Differentiation media Glioma differentiation into Human Neuronal lineage

Stem cells have the unique ability to self-renew or differentiate themselves into various cell types in response to appropriate signals. These cells are especially important for tissue repair, regeneration, replacement, or in the case of hematopoietic stem cells (HSCs) to differentiate into various myeloid populations. Appropriate signals refer to the growth factor supplements or cytokines that mediate differentiation of various stem cells into the required differentiated form. For instance, HSCs can be differentiated into dendritic cells (with IL-4 and GM-CSF), macrophages (with m-CSF) and MDSCs (with IL-6 and GM-CSF). Human pluripotent stem cells (hPSCs) and induced pluripotent stem cells (iPSCs) can be first cultured in neural differentiation media (GSK3𝛃-i, TGF𝛃-i, AMPK-i, hLIF) to form neural rosettes, which can be differentiated into neural or glial progenitors (finally differentiated into oligodendrocytes). Neural progenitors can be finally differentiated into glutaminergic (dibytyryl cAMP, ascorbic acid) and dopaminergic (SHH, FGF-8, BDNF, GDNF, TGF-𝛃3) neurons. Thus, it is important to first identify the self-renewing cell line: its source and its final differentiation state, followed by the supplements and cytokines required for the differentiation, and final use. Timelines are another thing that is considered. For instance, it takes 7-10 days to form neural rosettes from iPSCs and 3 days to differentiate neural progenitors to neurons. Finally, the stability for stem cell culture media varies. It is advised to make fresh media every time when differentiating HSCs to myeloid populations, whereas neural differentiation media may remain stable for two weeks when stored in dark between 2-8C.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 3 matching solutions for this experiment

Gibco™Neurobasal™ Medium

Thermo Fisher Scientific

Upstream tips
Culture vessels were coated with poly-l-lysine.
Protocol tips
Neurobasal medium (ThermoFisher, 21,103), B-27 supplement (ThermoFisher, 17,504), GlutaMax-I supplement (ThermoFisher, 35,050), dibutyryl cAMP (0.5 mM added on day 7 for three days) and primocin was used for NSCs.
Gibco™DMEM/F-12

Thermo Fisher Scientific

Protocol tips
DMEM/F-12 medium, reduced serum to 1% (v/v) FBS, 2% (v/v) Pen/Strep and supplemented with 10 µM of all-trans RA (differentiation medium)
Protocol tips
DMEM with only 1% FBS with RA [10 ΟM]
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms