Stem cell Differentiation media hDPSCs differentiation into chondrogenic cells

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Found 3 matching solutions for this experiment

Upstream tips
medium supplemented with 10 μg/ml ITS-X (Invitrogen), 5.35 μg/ml linoleic acid, 1.25 μg/ml bovine serum albumin, 1.0 μg/ml dexamethasone and 10 ng/ml TGF-β3 (Chemicon, Temecula, CA, USA)
Protocol tips
 α-Minimal Essential Medium (MEM) (Hyclone, Logan, UT, USA), 20% fetal bovine serum (Hyclone), 100 μM L-ascorbic acid-2-phosphate (Sigma, St Louis, MO, USA), 100 units/ml penicillin (Sigma) and 100 μg/ml streptomycin (Invitrogen)
Protocol tips
DMEM-HG supplemented with 5% FBS, 100 nM dexamethasone and 10 ng/ml TGFβ-3, 10 mM 2P-ascorbic acid (Sigma Aldrich, St. Louis, MO, United States), 1% v/v sodium pyruvate (Life Technologies, Carlsbad, CA, United States), 50 mg/ml ITS premix (BD Biosciences, Franklin Lakes, NJ, United States)
Protocol tips
αMEM supplemented with 1% penicillin and streptomycin, 10% FBS, 50 nmol/L ascorbic acid 2-phosphate, 6.25 mg/mL bovine insulin, and 10 ng/mL transforming growth factor–beta 1 (TGF-β1β1/ Sigma-Aldrich)
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