Stem cell Differentiation media hTSPCs differentiation into Chondrogenic cells

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Protocol tips
HG-DMEM supplemented with 1% of FBS, 2 mM L-glutamine, 50 U/mL penicillin, 50 mg/mL streptomycin, 1 mM sodium pyruvate (all from Sigma-Aldrich), 1% ITS + 1 (1.0 mg/mL insulin from bovine pancreas, 0.55 mg/mL human transferrin, 0.5 μg/mL sodium selenite, 50 mg/mL bovine serum albumin and 470 μg/mL linoleic acid, Sigma-Aldrich), 0.1 mM dexamethasone, 0.1 mM L-ascorbic acid-2-phosphate, 10 ng/ml TGF-β1 (Peprotech) 
Protocol tips
HG-DMEM supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 0.29 mg/ml L-glutamine, 1 mM sodium pyruvate, 1.25 mg/ml human serum albumin (HAS; Sigma-Aldrich), 1% ITS+1 (containing 1.0 mg/ml insulin from bovine pancreas, 0.55 mg/ml human transferrin, 0.5 μg/ml sodium selenite, 50 mg/ml bovine serum albumin and 470 μg/ml linoleic acid (Sigma-Aldrich), 0.1 μM dexamethasone, 0.1 mM L-ascorbic acid-2-phosphate, and 10 ng/ml TGF-β1 (PeproTech, Rocky Hill, NJ, USA)
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