Stem cell Differentiation media iPSCs or hESCs differentiation into Neuronal cells

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Found 3 matching solutions for this experiment

Protocol tips
DMEM/F12-GLUTAMAX-type medium containing 4% B27 supplement without vitamin A (Invitrogen), 1% N2 supplement (Invitrogen), and 55 M 2-mercaptoethanol (2-ME). To induce forebrain fate, the medium was supplemented with 5µM Y-27632 and 200ng/ml recombinant mouse Noggin (R&D Systems, 1967-NG-025)
Downstream tips
After 2 days, EBs were collected and plated onto 10-cm bacterial Petri dishes in 10 ml neuronal medium as above with the addition of B27 supplemented with vitamin A.

Thermo Fisher Scientific

Protocol tips
increasing amounts of N2 media (25, 50, 75, 100%) were added to the mTeSR medium every other day whilst maintaining 2.5uM dorsomorphin. N2 medium contains 1X N2 supplement (Invitrogen), 50% DMEM/F12, 50% Neurobasal, 0.075% Bovine serum albumin, and 2mM GlutaMAX (Life Technologies)
Downstream tips
Cells were subsequently passaged with trypsin and cultured on matrigel-coated dishes in N2 media with the addition of 20ng/ml bFGF. Rho-associated protein kinase (ROCK) inhibitor Y27632 (10uM, Stemgent) was added to the medium for the first 24 hours. NPs were expanded and maintained in N2 medium with 20ng/ml bFGF. All 2D culture experiments were performed on WIBR3-derived NPs unless otherwise specified.
Gibco™Neurobasal™ Medium

Thermo Fisher Scientific

Upstream tips
NS cells were grown on poly-L-ornithin and laminin coated plates. Poly-L-ornithine/laminin plates were generated as such: A 20 ug/ml solution of poly-L-ornithine (Sigma) in water was added to plates and plates were incubated at 37°C for 1 hour. The poly-L-ornithine solution was then removed, plates were washed three times with PBS, and then 5 ul/ml solution of laminin (Sigma) in PBS was added to plates and plates were incubated at 37°C for at least 3 hours.
Protocol tips
1:1 mix of DMEM/F12:Neurobasal (Invitrogen), 0.5X N2 (Invitrogen), 0.5X B27 (Invitrogen), 1X Glutamax, and 0.1 mM beta-mercaptoethanol, which was supplemented with 20 ng/ul of both EGF and FGF-2 (R&D)
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