Western blotting Rac1

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

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Found 3 matching solutions for this experiment

RAC1 Antibody

Proteintech Group

Protocol tips
-Rabbit (1:500)
-Soak PVDF membranewith pore size 0.45 um in methanol for 30 sec
-Apply Wet transfersystems at 180mA for 90 min with Wet transfer buffer.
RAC1 Polyclonal Antibody

Thermo Fisher Scientific

Protocol tips
-Rabbit (1:1000)
Rac1/2/3 Antibody #2465

Cell Signaling Technology

Protocol tips
-Rabbit (1:300)
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