Wound healing assay cell type - human HUVEC

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

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4 years ago

4 years ago by Lauren Dobson United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

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Protocol tips
- Cells are cultured/seeded in presence of EGM2S + for 18h..

- After cells are carefully washed, medium was then replaced with EGM2S +, EGM2S −, CMS, CMS + Dex (50 nM) or CMD
Protocol tips
- Cells are incubated for 4h.

- After 18h of creating a scratch cells are stained with Calcein AM (0.5 µg/ml)
Protocol tips
- Culture medium containing 5% FBS supplemented with 10 ng/mL VEGF. Cells are allowed to migrate for 4h.
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