gRNA_Cloning Vector

CRISPR Mouse - Deletion L929 Gβ2

Experiment
CRISPR Mouse - Deletion L929 Gβ2
Product
gRNA_Cloning Vector from Addgene
Manufacturer
Addgene

Protocol tips

Protocol tips
There is no need to perform a negative control golden-gate reaction (without
insert) as it will always contain colonies so not a good indicator of cloning success.

Publication protocol

CRISPR/Cas9-mediated gene knockout
The method used for CRISPR/Cas9-mediated gene knockout was described in35,36. In brief, Cas9-target sites for the indicated genes were designed in http://www.genome-engineering.org/crispr/?page_id=41. Then, synthetic nucleotides containing target sites were sub-cloned into gRNA_Cloning Vector (Addgene). Then gRNA vector was co-transfected with hCas9 (Addgene) into L929 cells. After G418 selection, cells were isolated as single clones. The protein quantity of each single clone was analyzed. The Cas9-target sites are: Gβ2-target site-1: TCATCTGAATTCGCCCCAC; Gβ2-target site-2: CTGTGCCTACGCCCCCTCA; Src-target site: GCCGCGGGCGGCACGAAGG.

Full paper   Login or join for free to view the full paper.

Reviews

gRNA_Cloning Vector from Addgene has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

4 years ago

Author: Mario Udinese Italy

Floxing mice with CRISPR

Hi everyone! I am planning on floxing mice with CRISPR but I am having trouble deciding which region to target. Do you have any tips on choosing?

Discussion

5 years ago

Author: Ben Saar Israel

How to choose a region to target for CRISPR

Hi everyone! I am planning on floxing mice with CRISPR but I am having trouble deciding which region to target. Do you have any tips on choosing?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing CRISPR Mouse - Deletion L929 Gβ2 using gRNA_Cloning Vector from Addgene.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Addgene for gRNA_Cloning Vector below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing CRISPR Mouse - Deletion L929 Gβ2 using gRNA_Cloning Vector from Addgene. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms