QuantiFluor® dsDNA System

DNA quantification Human - THP 1

Experiment
DNA quantification Human - THP 1
Product
QuantiFluor® dsDNA System from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
Exposure to light will reduce the sensitivity of the assay. Store QuantiFluor® dsDNA Dye and working solution protected from light

Publication protocol

Gene expression
RNA was extracted with TRI Reagent. In parallel to RNA, the double stranded DNA was quantified using Quantus™ Fluorometer and corresponding QuantiFluor® RNA and dsDNA Systems to exclude possible contamination of RNA with genomic DNA. The concentration of double stranded DNA was always below the detection limit. The isolated RNA was reversed transcribed (High Capacity cDNA Reverse Transcription Kit, Thermofisher Scientific) and cDNA was quantified by real-time PCR (Mic, Bio Molecular Systems) using primers listed in Supplem. Table 1 and Kapa Sybr Fast qPCR Master Mix. ACTB was used as a reference gene.

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Reviews

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Discussion

Discussion

5 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - THP 1 using QuantiFluor® dsDNA System from Promega.

Paper title
Downregulation of transcription by promoter-associated E2F4-RBL2-HDAC1-BRM complex contributes to repression of pluripotency stem cell factors in human monocytes
Full paper
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Manufacturer protocol

Download the product protocol from Promega for QuantiFluor® dsDNA System below.

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Videos

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