CelLytic™ B Plus Kit

Protein isolation Bacteria - Escherichia coli

Experiment
Protein isolation Bacteria - Escherichia coli
Product
CelLytic™ B Plus Kit from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- The protocol was followed according to manufacturer’s instructions.

Publication protocol

Cells were grown for 21 hours, reaching similar densities, harvested and lysed with CelLytic™ B Plus Kit (Sigma-Aldrich).

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Discussion

Discussion

5 years ago

Author: Hollie Fowler United Kingdom

How can I deal with my pellet being too viscous?

I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Bacteria - Escherichia coli using CelLytic™ B Plus Kit from Sigma-Aldrich.

Paper title
Genetic toolbox for controlled expression of functional proteins in spp.
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ B Plus Kit below.

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Videos

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