High Pure miRNA Isolation Kit

RNA isolation / purification Tissue - Human Bone marrow

Experiment
RNA isolation / purification Tissue - Human Bone marrow
Product
High Pure miRNA Isolation Kit from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step

Publication protocol

The Roche High Pure miRNA Isolation Kit (Catalogue #05080576001, Dee Why, NSW AUST) was used as per the manufacturer’s instructions where possible, however modifications to the protocol were needed to improve the total RNA yield

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Bone marrow using High Pure miRNA Isolation Kit from Sigma-Aldrich.

Paper title
Evaluation of MicroRNA Expression in Patient Bone Marrow Aspirate Slides
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for High Pure miRNA Isolation Kit below.

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Videos

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