SV Total RNA Isolation System

RNA isolation / purification Tissue - Human Breast

Experiment
RNA isolation / purification Tissue - Human Breast
Product
SV Total RNA Isolation System from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
- Do not freeze-thaw the DNase more than three times after rehydration.

Publication protocol

RNA was isolated using the SV Total RNA isolation kit (Promega). Verso cDNA synthesis kit and Absolute Blue SYBR Green ROX mix (Thermo Fisher Scientific Inc.) were used for RT-PCR. Real-time PCR (Rotor Gene 3000, Corbett) included amplification cycles of 5 sec at 95°c followed by 30 sec at 60°c.

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Breast using SV Total RNA Isolation System from Promega.

Paper title
Tissue Specific DNA Methylation in Normal Human Breast Epithelium and in Breast Cancer
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Manufacturer protocol

Download the product protocol from Promega for SV Total RNA Isolation System below.

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Videos

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