TRIzol Reagent

RNA isolation / purification Tissue - Human Hippocampus

Experiment
RNA isolation / purification Tissue - Human Hippocampus
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

To extract total RNA, 1 ml of TRIzol Reagent (Life Technologies, Foster City, CA, USA) was added per 75 mg of frozen tissue samples, homogenized and then further processed according to the manufacturer’s instructions. The RNA integrity number (RIN) mean in both the control and patient groups was similar: 6.68 ± 0.9441 (n = 5) and 6.155 ± 0.2484 (n = 11), respectively. Due to the fact that the RNA was unavailable, the RIN was not evaluated for three patient samples: TLE 03, TLE 11 and TLE 13. Subsequently, 1 μg of total RNA of each sample was reverse transcribed into cDNA using 200 U of Superscript III Reverse Transcriptase (Life Technologies) and 3 μg of Random Primers (Life Technologies) according to the manufacturer’s instructions.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Hippocampus using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Hippocampal gene expression dysregulation of Klotho, nuclear factor kappa B and tumor necrosis factor in temporal lobe epilepsy patients
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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