MagMAX™-96 Total RNA Isolation Kit

RNA isolation / purification Tissue - Human Ovaries

Experiment
RNA isolation / purification Tissue - Human Ovaries
Product
MagMAX™-96 Total RNA Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from ≤ 5 mg animal tissue.
Protocol tips
- To avoid DNA contamination, either reduce sample size, or include an additional DNase treatment step after RNA isolation
Downstream tips
- If there is low yield and/or inconsistent yield, lowering the amount of sample input may improve results

Publication protocol

Total RNA was isolated from the ovarian specimen and from theca-interstitial cells using the MagMAX-96 Total RNA Isolation Kit (Applied Biosystems) and the King Fisher robot (Thermo Scientific). Reverse transcription of total RNA to complementary DNA (cDNA) was performed using the High Capacity cDNA Reverse Transcription Kit for reverse transcription–polymerase chain reaction (RT-PCR) (Applied Biosystems). The PCR assays were set up in 28-µL volumes, consisting of 5 µL cDNA, 4.5 µL forward and 4.5 µL reverse 900n Mprimers, and 14 µL of 2× SYBR Green PCR Master Mix (Applied Biosystems).

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Ovaries using MagMAX™-96 Total RNA Isolation Kit from Thermo Fisher Scientific.

Paper title
Letrozole increases ovarian growth and gene expression in the rat ovary
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MagMAX™-96 Total RNA Isolation Kit below.

Download PDF Download manufacturer protocol

Videos

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