PicoPure™ RNA Isolation Kit

RNA isolation / purification Tissue - Human Pancreas

Experiment
RNA isolation / purification Tissue - Human Pancreas
Product
PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- Can be used when there is a very less amount of starting material

Publication protocol

Total RNA from each microdissected and lysed tissue was purified with the Arcturus PicoPure Frozen RNA Isolation Kit of Applied Biosystems and eluted in 11 μl, according to the protocol of the manufacturer.

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Pancreas using PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific.

Paper title
Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PicoPure™ RNA Isolation Kit below.

Download PDF Download manufacturer protocol

Videos

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