RNeasy Mini Kit

RNA isolation / purification Tissue - Mouse Pancreas

Experiment
RNA isolation / purification Tissue - Mouse Pancreas
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was extracted from pancreatic tissue using the RNeasy® mini kit (Qiagen, Inc., Valencia, CA, USA) and the concentration was determined using a SmartSpec Plus Spectrophotometer (BioRad, Hercules, CA, USA). First-strand cDNA was synthesized using 1 μg of total RNA in a 20 μl reverse transcriptase reaction mixture using iScript™ cDNA synthesis kit (BioRad) according to the manufacturer’s instructions. The region of tumor necrosis factor-α (Tnfa), interleukin-6 (Il6), monocyte chemoattractant protein-1 (Mcp1), vascular adhesion molecule 1 (Vcam1), sonic hedgehog (Shh) and Gli transcription factor (Gli) mRNA were amplified using a real-time PCR approach according to our previous published protocol (17, 18). All real-time PCR reactions and quantitations were performed using the MiniOpticon Real-Time PCR System (BioRad). Data of each mRNA expression are shown as the relative fold change normalized by that of glyceraldehyde 3-phosphate dehydrogenase (Gapdh).

Full paper   Login or join for free to view the full paper.

Reviews

RNeasy Mini Kit from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Pancreas using RNeasy Mini Kit from Qiagen.

Paper title
Inhibition of Chronic Pancreatitis and Murine Pancreatic Intraepithelial Neoplasia by a Dual Inhibitor of c-RAF and Soluble Epoxide Hydrolase in Mice
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Mouse Pancreas using RNeasy Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms