PicoPure™ RNA Isolation Kit

RNA isolation / purification Tissue - Rat Bronchi

Experiment
RNA isolation / purification Tissue - Rat Bronchi
Product
PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- Can be used when there is a very less amount of starting material

Publication protocol

Arcturus Picopure RNA extraction buffer (Applied Biosystems, Carlsbad, CA) was used the day of microdissection for lysing each sample. The samples were centrifuged briefly and kept at -80°C until the RNA isolation. RNA isolation was performed within five weeks of microdissection. RNA was extracted from normal tissue and PD-induced lesions. Picoscale RNA was isolated using the PicoPure RNA Isolation Kit (Molecular Devices, Sunnyvale, CA), adapting the protocol for use with CapSure LCM Caps. The amount of isolated RNA (pmole/μl) was determined with a Qubit Instrument. Isolated RNA was analyzed for quality and concentration using a Bioanalyzer PicoChip (Agilent Technologies, Englewood, CO). Twenty (20) ng of total RNA was amplified and labeled using the Nugen WT-Ovation Pico RNA Amplification and Encore Biotin Module protocols.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Bronchi using PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific.

Paper title
Gene Expression in Obliterative Bronchiolitis-Like Lesions in 2,3-Pentanedione-Exposed Rats
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PicoPure™ RNA Isolation Kit below.

Download PDF Download manufacturer protocol

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