APO-BRDU™ Kit (RUO)

TUNEL assay cell type - Rabbit synovial fibroblasts

Experiment
TUNEL assay cell type - Rabbit synovial fibroblasts
Product
APO-BRDU™ Kit (RUO) from BD Biosciences
Manufacturer
BD Biosciences

Protocol tips

Upstream tips
- 1.5×10^5 cells were platted in 6cm culture dish
Protocol tips
- Cells were fixed in 1% paraformaldehyde in PBS for 30 min.

- Dehydrate cells in 70% methanol at 4°C for 30 min.

Publication protocol

HIG-82 synoviocytes (1.5×105) were plated in 6-cm culture dishes for 24 h. The culture medium was replaced with three types of media: (1) FC medium, (2) MFD medium and (3) FD medium for 48 h. Cells were fixed in 1% paraformaldehyde in PBS for 30 min, then washed with PBS, and stored in 70% methanol at 4°C. After rehydration in PBS, cells were assayed with Apoptosis Detection Kit (APO-BRDU) (BD Pharmigen) [29]. The data were acquired, analyzed and plotted by the CellQuest Pro software and the Sigma Plot 10.0 software.

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - Rabbit synovial fibroblasts using APO-BRDU™ Kit (RUO) from BD Biosciences.

Paper title
Folate Deficiency Triggered Apoptosis of Synoviocytes: Role of Overproduction of Reactive Oxygen Species Generated via NADPH Oxidase/Mitochondrial Complex II and Calcium Perturbation
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Manufacturer protocol

Download the product protocol from BD Biosciences for APO-BRDU™ Kit (RUO) below.

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