Cell Death Detection ELISA

TUNEL assay cell type - Rat pulmonary arterial smooth muscle cells

Experiment
TUNEL assay cell type - Rat pulmonary arterial smooth muscle cells
Product
Cell Death Detection ELISA from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- Slides were fixed with 4% paraformaldehyde for 30 min at room temperature.

- Slides were permeabilized with 1% Triton X-100 for 4 min at 4°C instead of 2min on ice.

- Slides were labelled at 37°C for 60 min in a dark environment

Publication protocol

DNA fragmentation in PASMCs was measured using the TUNEL assay (Cell Death Detection Kit, Roche Biochemicals, Mannheim, Germany). Briefly, air-dried slides were fixed with 4% paraformaldehyde for 30 min at room temperature, cleaned three times with PBS for 10 min, and then permeabilized with 1% Triton X-100 for 4 min at 4°C. Then, the TdT-labelled nucleotide mix was added to each slide and incubated at 37°C for 60 min in a dark environment. The slides were washed twice with PBS and then counterstained with 10 mg/ml 4,6-diamidino-2-phenylindole (DAPI) for 5 min at 37°C [23].

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - Rat pulmonary arterial smooth muscle cells using Cell Death Detection ELISA from Sigma-Aldrich.

Paper title
Resveratrol Protects Against Pulmonary Arterial Hypertension in Rats via Activation of Silent Information Regulator 1.
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Cell Death Detection ELISA below.

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