DeadEnd™ Fluorometric TUNEL System

TUNEL assay cell type - SK-MEL-2 human melanoma

Experiment
TUNEL assay cell type - SK-MEL-2 human melanoma
Product
DeadEnd™ Fluorometric TUNEL System from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
1065 cells/well
Protocol tips
Transfect for 48h
Downstream tips
- When there was a little or poor staining, optimise permeabilization step
by adjusting incubation time with permeabilization agent.


- When there is a loss of tissue from slides, decrease
Proteinase K incubation time.

Publication protocol

DNA fragmentation was evaluated by the DeadEndTM Fluorometric TUNEL kit (Promega) according to the manufacturer's directions and flow cytometric quantification of hypodiploid cells (sub-G1 fraction). For quantification of hypodiploid events, 105 cells/well were transfected for 48 h as described above. Staurosporine (STP, Sigma) was used at a concentration of 5 μM as a positive control of apoptosis. Cells were harvested and centrifuged at 600 × g for 5 min. Pellets were suspended in 100% ethanol and stored at −20 °C for 24 h. Cells were then treated with 1 mg/ml RNase A for 1 h at RT. PI was added, and samples were subjected to flow cytometry.

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Reviews

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - SK-MEL-2 human melanoma using DeadEnd™ Fluorometric TUNEL System from Promega.

Paper title
Kinase inhibitor screening identifies CDK4 as a potential therapeutic target for melanoma
Full paper
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Manufacturer protocol

Download the product protocol from Promega for DeadEnd™ Fluorometric TUNEL System below.

Download PDF Download manufacturer protocol

Videos

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