ChIP H3K4Me3 Goat

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using Histone H3K4me3 antibody (mAb) to perform ChIP Anti-bodies H3K4me3

Get tips on using Histone H3K4me3 antibody (mAb) to perform ChIP Anti-bodies H3K27me1

Get tips on using Goat Anti-Type III Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-

Get tips on using Goat Anti-Type I Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-

Get tips on using Goat antibody to Calretinin to perform Immunohistochemistry Mouse - Calb2

Get tips on using Goat Anti-Type III Collagen-BIOT to perform Immunohistochemistry Collagen Type III - Goat Mouse Biotin

Get tips on using Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate to perform Western blot Secondary Antibody - Goat Mouse Horseradish peroxidase

Get tips on using Goat Anti-Rabbit IgG (H + L)-HRP Conjugate to perform Western blot Secondary Antibody - Goat Rabbit Horseradish peroxidase

Get tips on using Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580) to perform ChIP Anti-bodies H3K4me3