ChIP H3K9me3 Hamster

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Get tips on using Histone H3K9me3 antibody (mAb) to perform ChIP Anti-bodies H3K9me3

Get tips on using Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) to perform ChIP Anti-bodies H3K9me3

Get tips on using Purified Hamster Anti-Mouse CD80 to perform Flow cytometry Anti-bodies Mouse - CD80

Get tips on using FITC Hamster Anti-Mouse CD40 to perform Flow cytometry Anti-bodies Mouse - CD40

Get tips on using PE Hamster Anti-Mouse CD279 to perform Flow cytometry Anti-bodies Mouse - CD279/PD-1

Get tips on using Purified Hamster Anti-Mouse TCR β Chain to perform Flow cytometry Anti-bodies Mouse - TCRbeta

Get tips on using Purified NA/LE Hamster Anti-Mouse CD40 to perform Flow cytometry Anti-bodies Mouse - CD40

Get tips on using PerCP-Cy™5.5 Hamster Anti-Mouse CD69 to perform Flow cytometry Anti-bodies Mouse - CD69

Get tips on using PE-Cy™7 Hamster Anti-Mouse CD11c to perform Flow cytometry Anti-bodies Mouse - CD11c