Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor DNA Site Directed Mutagenesis (SDM) Mouse C2C12 myogenin Site Directed Mutagenesis (SDM) Mouse - C2C12 myogenin Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template. 1 Matching solution Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 1 matching solution for this experiment GeneArt™ Site-Directed Mutagenesis System Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Upstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. Protocol tips - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Manufacturer protocol Publication protocol Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor DNA Site Directed Mutagenesis (SDM) Mouse Neuroblastoma 2a dibasic site, R18Q Site Directed Mutagenesis (SDM) Mouse - Neuroblastoma 2a dibasic site, R18Q Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template. 1 Matching solution Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 1 matching solution for this experiment QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn Agilent Technologies Upstream tips Protocol tips Downstream tips - Use only the Dpn I enzyme provided; do not substitute with an enzyme from another source. Protocol tips - Use only the Dpn I enzyme provided; do not substitute with an enzyme from another source. Manufacturer protocol Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor DNA Site Directed Mutagenesis (SDM) Mouse L929 T1L σ1 Site Directed Mutagenesis (SDM) Mouse - L929 T1L σ1 Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template. 1 Matching solution Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 1 matching solution for this experiment GeneArt™ Site-Directed Mutagenesis PLUS System Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Upstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. Protocol tips - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Manufacturer protocol Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor DNA Site Directed Mutagenesis (SDM) Mouse Neuroblastoma 2a Epac1 Site Directed Mutagenesis (SDM) Mouse - Neuroblastoma 2a Epac1 Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template. 1 Matching solution Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 1 matching solution for this experiment GeneArt™ Site-Directed Mutagenesis System Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Upstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. Protocol tips - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Manufacturer protocol Publication protocol Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor DNA Site Directed Mutagenesis (SDM) Human Deletion PC-3 AGR3 Site Directed Mutagenesis (SDM) Human - Deletion PC-3 AGR3 Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template. 2 Matching solutions Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 2 matching solutions for this experiment Q5® Site-Directed Mutagenesis Kit New England BioLabs Upstream tips Protocol tips Downstream tips - Only use 1 μl of PCR product in the KLD reaction. Carrying too much PCR product forward can decrease transformation efficiency. - Only use 5 μl of the KLD reaction in the transformation. If more KLD reaction is added, a buffer exchange step, such as PCR purification, should be included prior to transformation. Protocol tips - Only use 1 μl of PCR product in the KLD reaction. Carrying too much PCR product forward can decrease transformation efficiency. - Only use 5 μl of the KLD reaction in the transformation. If more KLD reaction is added, a buffer exchange step, such as PCR purification, should be included prior to transformation. Manufacturer protocol Publication protocol 1 Relevant paper QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn Agilent Technologies Upstream tips Protocol tips Downstream tips - Use only the Dpn I enzyme provided; do not substitute with an enzyme from another source. Protocol tips - Use only the Dpn I enzyme provided; do not substitute with an enzyme from another source. Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor DNA Site Directed Mutagenesis (SDM) Human Deletion PC-3 AGR2 Site Directed Mutagenesis (SDM) Human - Deletion PC-3 AGR2 Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template. 2 Matching solutions Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 2 matching solutions for this experiment Q5® Site-Directed Mutagenesis Kit New England BioLabs Upstream tips Protocol tips Downstream tips - Only use 1 μl of PCR product in the KLD reaction. Carrying too much PCR product forward can decrease transformation efficiency. - Only use 5 μl of the KLD reaction in the transformation. If more KLD reaction is added, a buffer exchange step, such as PCR purification, should be included prior to transformation. Protocol tips - Only use 1 μl of PCR product in the KLD reaction. Carrying too much PCR product forward can decrease transformation efficiency. - Only use 5 μl of the KLD reaction in the transformation. If more KLD reaction is added, a buffer exchange step, such as PCR purification, should be included prior to transformation. Manufacturer protocol Publication protocol 1 Relevant paper QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn Agilent Technologies Upstream tips Protocol tips Downstream tips - Use only the Dpn I enzyme provided; do not substitute with an enzyme from another source. Protocol tips - Use only the Dpn I enzyme provided; do not substitute with an enzyme from another source. Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor DNA Site Directed Mutagenesis (SDM) Human Point mutation LNCaP RasGRP2 Site Directed Mutagenesis (SDM) Human - Point mutation LNCaP RasGRP2 Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template. 1 Matching solution Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 1 matching solution for this experiment GeneArt™ Site-Directed Mutagenesis System Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Upstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. Protocol tips - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor DNA Site Directed Mutagenesis (SDM) Human Insertion SKOV3 miR-134 Site Directed Mutagenesis (SDM) Human - Insertion SKOV3 miR-134 Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template. 1 Matching solution Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 1 matching solution for this experiment GeneArt™ Site-Directed Mutagenesis PLUS System Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Upstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. Protocol tips - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor DNA Site Directed Mutagenesis (SDM) Mouse Point mutation C2C12 myogenin Site Directed Mutagenesis (SDM) Mouse - Point mutation C2C12 myogenin Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template. 1 Matching solution Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 1 matching solution for this experiment GeneArt™ Site-Directed Mutagenesis System Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Upstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. Protocol tips - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor DNA Site Directed Mutagenesis (SDM) Dog Point mutation MDCK SCRIB Site Directed Mutagenesis (SDM) Dog - Point mutation MDCK SCRIB Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template. 1 Matching solution Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 1 matching solution for this experiment GeneArt™ Site-Directed Mutagenesis System Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Upstream tips - Use 20–25 ng of the target plasmid per 50 µL of mutagenesis reaction as a starting point. Protocol tips - Create a fresh dilution of 25X SAM from the kit-supplied 200X SAM in sterile, distilled water each time you perform the mutagenesis procedure - 25X SAM is not stable, and loses activity within a few hours after preparation Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms