Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3) CD24 lentiviral particles shRNA gene silencing Mouse - Prostate cancer cell lines (DU145 and PC3) CD24 lentiviral particles Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include: 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi have been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining efficacy of transduction and shRNA on its target site. 2 Matching solutions 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 8 months ago 8 months ago by A.C.Burton Multiple gene silencing using ShRNA Hello, can someone here help me? I am trying to silence e-selectin and ICAM-1 in endothelial cells. I would like to know if this is possible using shRNA Comment View 1 comment Share your thoughts or question with experts in your field by adding a discussion! Found 2 matching solutions for this experiment ShRNA CD24 Lentiviral Transduction Particles (CD24-V2LHS_71908) Dharmacon (GE Life Sciences) Upstream tips Protocol tips Downstream tips Seed cells in 10-cm plates and cultured with puromycin Human CD24-shRNA S1 TGCCTCGACACACATAAAC Human CD24- shRNA S2 TTGCATTGACCACGACTAA Upstream tips Seed cells in 10-cm plates and cultured with puromycin Protocol tips Human CD24-shRNA S1 TGCCTCGACACACATAAAC Human CD24- shRNA S2 TTGCATTGACCACGACTAA Manufacturer protocol Publication protocol 1 Relevant paper ShRNA CD24 Lentiviral Transduction Particles (CD24-V2LHS_71909)) Dharmacon (GE Life Sciences) Upstream tips Protocol tips Downstream tips Seed cells in 10-cm plates and cultured with puromycin Human CD24-shRNA S1 TGCCTCGACACACATAAAC Human CD24- shRNA S2 TTGCATTGACCACGACTAA Upstream tips Seed cells in 10-cm plates and cultured with puromycin Protocol tips Human CD24-shRNA S1 TGCCTCGACACACATAAAC Human CD24- shRNA S2 TTGCATTGACCACGACTAA Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA shRNA gene silencing Human Islets of langerhans SOX2 lentiviral particles shRNA gene silencing Human - Islets of langerhans SOX2 lentiviral particles Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include: 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi have been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining efficacy of transduction and shRNA on its target site. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 1 year ago 1 year ago by Eufrosina Sagese Is a knockdown using shRNA permanent? Is a knockdown using shRNA permanent and if not is there a known duration? Comment View 3 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment MISSION® shRNA SOX2 Lentiviral Transduction Particles Sigma-Aldrich Upstream tips Protocol tips Downstream tips Infect at MOI of 3:1 in CMRL 1066 medium containing 8 μg/ml polybrene for overnight. After 4 days select cells with 1 μg/ml puromycin for 3 days Protocol tips Infect at MOI of 3:1 in CMRL 1066 medium containing 8 μg/ml polybrene for overnight. After 4 days select cells with 1 μg/ml puromycin for 3 days Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA shRNA gene silencing Human Islets of langerhans ZEB1 lentiviral particles shRNA gene silencing Human - Islets of langerhans ZEB1 lentiviral particles Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include: 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi have been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining efficacy of transduction and shRNA on its target site. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 1 year ago 1 year ago by Eufrosina Sagese Is a knockdown using shRNA permanent? Is a knockdown using shRNA permanent and if not is there a known duration? Comment View 3 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment MISSION® shRNA ZEB1 Lentiviral Transduction Particles Sigma-Aldrich Upstream tips Protocol tips Downstream tips Infect at MOI of 3:1 in CMRL 1066 medium containing 8 μg/ml polybrene for overnight. After 4 days select cells with 1 μg/ml puromycin for 3 days. Protocol tips Infect at MOI of 3:1 in CMRL 1066 medium containing 8 μg/ml polybrene for overnight. After 4 days select cells with 1 μg/ml puromycin for 3 days. Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA shRNA gene silencing Human Islets of langerhans SOX6 lentiviral particles shRNA gene silencing Human - Islets of langerhans SOX6 lentiviral particles Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include: 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi have been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining efficacy of transduction and shRNA on its target site. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 1 year ago 1 year ago by Eufrosina Sagese Is a knockdown using shRNA permanent? Is a knockdown using shRNA permanent and if not is there a known duration? Comment View 3 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment MISSION® shRNA SOX6 Lentiviral Transduction Particles Sigma-Aldrich Upstream tips Protocol tips Downstream tips Infect at MOI of 3:1 in CMRL 1066 medium containing 8 μg/ml polybrene for overnight. After 4 days select cells with 1 μg/ml puromycin for 3 days Protocol tips Infect at MOI of 3:1 in CMRL 1066 medium containing 8 μg/ml polybrene for overnight. After 4 days select cells with 1 μg/ml puromycin for 3 days Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA shRNA gene silencing Human Islets of langerhans ZEB2 lentiviral particles shRNA gene silencing Human - Islets of langerhans ZEB2 lentiviral particles Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include: 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi have been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining efficacy of transduction and shRNA on its target site. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 1 year ago 1 year ago by Eufrosina Sagese Is a knockdown using shRNA permanent? Is a knockdown using shRNA permanent and if not is there a known duration? Comment View 3 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment MISSION® shRNA ZEB2 Lentiviral Transduction Particles Sigma-Aldrich Upstream tips Protocol tips Downstream tips Infect at MOI of 3:1 in CMRL 1066 medium containing 8 μg/ml polybrene for overnight. After 4 days select cells with 1 μg/ml puromycin for 3 days Protocol tips Infect at MOI of 3:1 in CMRL 1066 medium containing 8 μg/ml polybrene for overnight. After 4 days select cells with 1 μg/ml puromycin for 3 days Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA shRNA gene silencing Human Islets of langerhans Negative control (scrambled) lentiviral particles shRNA gene silencing Human - Islets of langerhans Negative control (scrambled) lentiviral particles Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include: 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi have been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining efficacy of transduction and shRNA on its target site. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 1 year ago 1 year ago by Eufrosina Sagese Is a knockdown using shRNA permanent? Is a knockdown using shRNA permanent and if not is there a known duration? Comment View 3 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles Sigma-Aldrich Upstream tips Protocol tips Downstream tips Infect at MOI of 3:1 in CMRL 1066 medium containing 8 μg/ml polybrene for overnight. After 4 days select cells with 1 μg/ml puromycin for 3 days Protocol tips Infect at MOI of 3:1 in CMRL 1066 medium containing 8 μg/ml polybrene for overnight. After 4 days select cells with 1 μg/ml puromycin for 3 days Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA shRNA gene silencing Human HEK293T CAPN5- (Calpains) cationic lipid based shRNA gene silencing Human - HEK293T CAPN5- (Calpains) cationic lipid based Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include: 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi have been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining efficacy of transduction and shRNA on its target site. 2 Matching solutions 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 1 year ago 1 year ago by Eufrosina Sagese Is a knockdown using shRNA permanent? Is a knockdown using shRNA permanent and if not is there a known duration? Comment View 3 comments Share your thoughts or question with experts in your field by adding a discussion! Found 2 matching solutions for this experiment Attractene Transfection Reagent Qiagen Upstream tips Protocol tips Downstream tips Seed 5.0 × 10^5 cells Add 4.5 μL Attractene Transfection Reagent to the DNA solution. Incubate the cells under their normal growth conditions and change medium after 6h and continue to incubate until 48 h Upstream tips Seed 5.0 × 10^5 cells Protocol tips Add 4.5 μL Attractene Transfection Reagent to the DNA solution. Incubate the cells under their normal growth conditions and change medium after 6h and continue to incubate until 48 h Manufacturer protocol Publication protocol 1 Relevant paper Target sequence1: Seq1-5'-GACTTCACGGGTGGTGTTTCT-3' Custom made Upstream tips Protocol tips Downstream tips Seed 5.0 × 10^5 cells Add 4.5 μL Attractene Transfection Reagent to the DNA solution. Incubate the cells under their normal growth conditions and change medium after 6h and continue to incubate until 48 h Upstream tips Seed 5.0 × 10^5 cells Protocol tips Add 4.5 μL Attractene Transfection Reagent to the DNA solution. Incubate the cells under their normal growth conditions and change medium after 6h and continue to incubate until 48 h Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA shRNA gene silencing Human Neuroblastoma cells (SH-SY5Y) Connexin 43 lentiviral particles shRNA gene silencing Human - Neuroblastoma cells (SH-SY5Y) Connexin 43 lentiviral particles Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include: 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi have been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining efficacy of transduction and shRNA on its target site. 1 Matching solution 1 Discussion Start discussion Found 1 discussion for this experiment Discussion 1 year ago 1 year ago by Eufrosina Sagese Is a knockdown using shRNA permanent? Is a knockdown using shRNA permanent and if not is there a known duration? Comment View 3 comments Share your thoughts or question with experts in your field by adding a discussion! Found 1 matching solution for this experiment connexin 43 ShRNA + Lipofectamine® 2000 Transfection Reagent connexin 43 ShRNA Santa Cruz Biotechnology Lipofectamine® 2000 Transfection Reagent Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips Add 1:1 ratio of DNA to diluted Lipofectamine® 2000 Reagent and Incubate for 5 minutes at room temperature. Incubate cells for 2 days at 37°C then analyze transfected cells Protocol tips Add 1:1 ratio of DNA to diluted Lipofectamine® 2000 Reagent and Incubate for 5 minutes at room temperature. Incubate cells for 2 days at 37°C then analyze transfected cells Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor Discussions Multiple gene silencing using ShRNA Discussion 8 months ago A.C.Burton 1 1 Multiple gene silencing using ShRNA Hello, can someone here help me? I am trying to silence e-selectin and ICAM-1 in endothelial cells. I would like to know if this is possible using shRNA Experiment: Comment 1 Comment Answer 1 Hello Burton, Generally it should be possible to target multiple genes however all of your constructs will contain the same selection marker. As a result, it would be impossible to determine which cells within your culture got both of the virus types. Answered 7 months ago S Janardhan Can you help? Add your comment Start your discussion Share your thoughts or question with experts in your field Start discussion Become shareholder Discussions About us Contact Privacy Terms

Find the best product for any wet lab experiment Shared knowledge is peer reviewed Ask questions, get answers Toggle navigation Experiments Products Discussions   Login   Join for free Labettor RNA siRNA / miRNA gene silencing Human LNCap PIN1 siRNA / miRNA gene silencing Human - LNCap PIN1 Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells. 1 Matching solution Start a discussion Start discussion No discussions found Start your discussion Share your thoughts or question with experts in your field Start a discussion Found 1 matching solution for this experiment siRNA PIN1 + Lipofectamine® RNAiMAX Transfection Reagent siRNA PIN1 Thermo Fisher Scientific Lipofectamine® RNAiMAX Transfection Reagent Thermo Fisher Scientific Upstream tips Protocol tips Downstream tips Pin1 shRNA-1: 5’-CGGCAACAGCAGCAGUGGUGGCAAA-3’ Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 1–3 days at 37°C. Protocol tips Pin1 shRNA-1: 5’-CGGCAACAGCAGCAGUGGUGGCAAA-3’ Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 1–3 days at 37°C. Manufacturer protocol Publication protocol 1 Relevant paper Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product! Become shareholder Discussions About us Contact Privacy Terms