3D Cell Culture Media Human blood-brain barrier organoid

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Protocol tips
To reduce the ‘edge effect’ in a 96-well plate: fill the outer edge wells with 200 μL D-PBS or BBB working medium, use only the inner wells of the plate for culturing the BBB organoids
Protocol tips
Use of a combination of mediums for the organoid medium: 60% neural maintenance-XF medium, 20% astrocyte medium and 20% complete classic medium
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