Angiogenesis assay human - PMVEC

A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.

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5 years ago

5 years ago by Sander J. Mikkelsen Denmark

endothelial cells vs HUVEC cells?

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

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Protocol tips
- Plates are coated with Reduced Growth Factor Basement Membrane Extract and observed by microscopy after 16h.
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