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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| - Lyse cells with RIPA lysis buffer containing Tris-HCl 10 mmol/L, EDTA 5 mmol/L, NaCl 50 mmol/L, 1% deoxycholic acid and 1% triton X-100, pH 7.4 |
- Dilute Primary Ab -1:2000. |
|
| Upstream tips |
| - Lyse cells with RIPA lysis buffer containing Tris-HCl 10 mmol/L, EDTA 5 mmol/L, NaCl 50 mmol/L, 1% deoxycholic acid and 1% triton X-100, pH 7.4 |
| Protocol tips |
| - Dilute Primary Ab -1:2000. |
| Upstream tips |
Protocol tips |
Downstream tips |
| - Lyse cells on ice with RIPA buffer |
- Dilute Primary Ab -1:1000 dilution and incubate at 4°C overnight |
|
| Upstream tips |
| - Lyse cells on ice with RIPA buffer |
| Protocol tips |
| - Dilute Primary Ab -1:1000 dilution and incubate at 4°C overnight |
| Upstream tips |
Protocol tips |
Downstream tips |
|
Primary Ab dilution-(1:200) in PBS containing 10% goat serum |
|
| Protocol tips |
| Primary Ab dilution-(1:200) in PBS containing 10% goat serum |
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