No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 8 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Chloroquine, a well-known reagent able to block autophagic flux and cause an accumulation of autophagosome in the cytoplasm, is used to treat Caco2 cells to use as positive control. |
|
|
| Upstream tips |
| Chloroquine, a well-known reagent able to block autophagic flux and cause an accumulation of autophagosome in the cytoplasm, is used to treat Caco2 cells to use as positive control. |
| Upstream tips |
Protocol tips |
Downstream tips |
|
- dilute primary antibody at 1:1000 in 5% w/v BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
|
|
| Protocol tips |
- dilute primary antibody at 1:1000 in 5% w/v BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
|
| Upstream tips |
Protocol tips |
Downstream tips |
|
- dilute primary antibody at 1:1000 in 5% w/v BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
|
|
| Protocol tips |
- dilute primary antibody at 1:1000 in 5% w/v BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
|
| Upstream tips |
Protocol tips |
Downstream tips |
|
- For IHC, use promary Ab dilution at 1:600 and for Western Blot use between 1:1,000 – 1:3,000 |
|
| Protocol tips |
| - For IHC, use promary Ab dilution at 1:600 and for Western Blot use between 1:1,000 – 1:3,000 |
| Upstream tips |
Protocol tips |
Downstream tips |
| - Lyse cells in M-PER Mammalian Protein Extraction Reagent |
- Use a dilution range 1:1001:1000 |
|
| Upstream tips |
| - Lyse cells in M-PER Mammalian Protein Extraction Reagent |
| Protocol tips |
| - Use a dilution range 1:1001:1000 |
| Upstream tips |
Protocol tips |
Downstream tips |
| - Lyse cells in M-PER Mammalian Protein Extraction Reagent |
- Use a dilution range 1:1001:1000 |
|
| Upstream tips |
| - Lyse cells in M-PER Mammalian Protein Extraction Reagent |
| Protocol tips |
| - Use a dilution range 1:1001:1000 |
| Upstream tips |
Protocol tips |
Downstream tips |
| - Lyse cells in M-PER Mammalian Protein Extraction Reagent |
- Use a dilution range 1:1001:1000 |
|
| Upstream tips |
| - Lyse cells in M-PER Mammalian Protein Extraction Reagent |
| Protocol tips |
| - Use a dilution range 1:1001:1000 |
| Upstream tips |
Protocol tips |
Downstream tips |
|
- Use primary Ab dilution at 1:200 |
|
| Protocol tips |
| - Use primary Ab dilution at 1:200 |
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!