Bacterial cell culture media Lactobacillus helveticus

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

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5 years ago

5 years ago by Ghalia Hayat Qatar

Tomato juice medium

I am having trouble growing Lactobacillus helveticus in a tomato juice medium. Here is the formula that I am currently using for 400ml: Triptone 4g yeast extract 4g 80ml of filtrated tomato juice What might be the problem?

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Protocol tips
-pH at 25 °C pH6.5 ± 0.2.
-Incubation Temperature 30–35 °C.
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