Cell cytotoxicity / Proliferation assay cell type - HCT116

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Found 1 matching solution for this experiment

Upstream tips
Easy-to-use: There is no requirement for additional reagents
and/or the cell washing procedures
Speed: Multiwell plates and an ELISA reader can be used for
reading
Sensitivity: Can be assayed even in low cell concentrations
Accuracy: Dye absorbance is proportional to the number of
cells in each well
Safety: There is no need for radioactive isotopes
Protocol tips
Kit was developed for cell proliferation assay in reaction to different growth factors, cytokines and nutrient components.
It is suitable for assaying cytotoxicity of materials such as TNF or other growth inhibitors and for lymphocyte activation.
Kit is an excellent solution for the quantitating of cell proliferation and viability, without using radioactive isotopes also for non-radioactive substitute for cytotoxic tests based on the release of 51Cr from cells, maintaining high sensitivity.
Downstream tips
XTT results were blotted
and the dose needed to kill 50% of the cells was determined using Prism software.
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