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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Seed 1×10^4 cells per well. |
Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 24 hours (cell line dependent) to permit cell attachment |
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| Upstream tips |
| Seed 1×10^4 cells per well. |
| Protocol tips |
| Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 24 hours (cell line dependent) to permit cell attachment |
| Upstream tips |
Protocol tips |
Downstream tips |
| Prepare a cell suspension containing 0.5-1.0 x 106 cells/ml in serum free media |
Incubate for 2-24 hours in a cell culture incubator. |
Read fluorescence with a fluorescence plate reader at 480 nm/520 nm. |
| Upstream tips |
| Prepare a cell suspension containing 0.5-1.0 x 106 cells/ml in serum free media |
| Protocol tips |
| Incubate for 2-24 hours in a cell culture incubator. |
| Downstream tips |
| Read fluorescence with a fluorescence plate reader at 480 nm/520 nm. |
| Upstream tips |
Protocol tips |
Downstream tips |
| Add 1x10^5 endothelial cells |
Incubate 24 hrs in the cell incubator. |
Read plate at absorbance 540 – 570 nm |
| Upstream tips |
| Add 1x10^5 endothelial cells |
| Protocol tips |
| Incubate 24 hrs in the cell incubator. |
| Downstream tips |
| Read plate at absorbance 540 – 570 nm |
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