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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
Bring all reagents to room temperature (18-25°C) before use for 30min.
-Prepare fresh standard for each assay. Use within 4 hours and discard after use.
-Centrifuge the sample again after thawing before the assay. |
-To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps.
-Once reagents have been
added to the well strips, DO NOT let the strips DRY at any time during the assay.
-TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light.
-Stop Solution should be added to the plate in the same order as the TMB Substrate. |
|
| Upstream tips |
Bring all reagents to room temperature (18-25°C) before use for 30min.
-Prepare fresh standard for each assay. Use within 4 hours and discard after use.
-Centrifuge the sample again after thawing before the assay. |
| Protocol tips |
-To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps.
-Once reagents have been
added to the well strips, DO NOT let the strips DRY at any time during the assay.
-TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light.
-Stop Solution should be added to the plate in the same order as the TMB Substrate. |
| Upstream tips |
Protocol tips |
Downstream tips |
-All components of this kit except the standard are stable at 4˚C.
The standard must be stored at or below -20˚C.
-Bring all reagents except the
standard and assay buffer to
room temperature for at least
30 minutes prior to opening. |
-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation.
-Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results. |
|
| Upstream tips |
-All components of this kit except the standard are stable at 4˚C.
The standard must be stored at or below -20˚C.
-Bring all reagents except the
standard and assay buffer to
room temperature for at least
30 minutes prior to opening. |
| Protocol tips |
-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation.
-Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
|
| Upstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
| Protocol tips |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
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