Mammalian cell culture media CHO

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 4 matching solutions for this experiment

RPMI 1640

Nacalai Tesque

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific Inc.), 100 units/mL of penicillin, 100 μg/mL of streptomycin, and 25 μg/mL of amphotericin B (Nacalai Tesque, Inc.)

Protocol tips
supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific Inc.), 100 units/mL of penicillin, 100 μg/mL of streptomycin, and 25 μg/mL of amphotericin B (Nacalai Tesque, Inc.)

Publication protocol 1 Relevant paper

Dulbecco’s Modified Eagle’s Medium - high glucose

Sigma-Aldrich

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	with 10% fetal calf serum (Sigma-Aldrich, St. Louis, MO) and 1% antibiotic-antimitotic solution (Sigma-Aldrich, St. Louis, MO)

Protocol tips
with 10% fetal calf serum (Sigma-Aldrich, St. Louis, MO) and 1% antibiotic-antimitotic solution (Sigma-Aldrich, St. Louis, MO)

Manufacturer protocol Publication protocol 1 Relevant paper

CD CHO Medium

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	CD-CHO medium containing 8 mM L-glutamine

Protocol tips
CD-CHO medium containing 8 mM L-glutamine

Manufacturer protocol Publication protocol 1 Relevant paper

CHO Media

SARTORIUS

Upstream tips	Protocol tips	Downstream tips
Optimized for either stable or transient production 100% chemically defined, free of animal components, serum and hydrolysates. Liquid and powder formulations are available. Little or no adaptation from other serum-free media is needed. Robust performance in batch, fed-batch and perfusion cultivations. Customized solutions are available.	The media and feeds are ideal for protein production using CHO-DG44, CHO-K1, CHO-S and CHO-GS cell lines and are suitable for research and routine large-scale GMP manufacturing.

Upstream tips
Optimized for either stable or transient production 100% chemically defined, free of animal components, serum and hydrolysates. Liquid and powder formulations are available. Little or no adaptation from other serum-free media is needed. Robust performance in batch, fed-batch and perfusion cultivations. Customized solutions are available.
Protocol tips
The media and feeds are ideal for protein production using CHO-DG44, CHO-K1, CHO-S and CHO-GS cell lines and are suitable for research and routine large-scale GMP manufacturing.

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