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Experimental details for studies in primary human carotid artery smooth muscle cells (SMCs) and endothelial cells (HCtASMCs and human carotid artery endothelial cells; Cell Applications), using the Streamer apparatus (Flexcell International Corporation, Burlington, VT) with adjustable flow and shear stress conditions are described in the Methods in the Online Data Supplement. |
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Protocol tips |
Experimental details for studies in primary human carotid artery smooth muscle cells (SMCs) and endothelial cells (HCtASMCs and human carotid artery endothelial cells; Cell Applications), using the Streamer apparatus (Flexcell International Corporation, Burlington, VT) with adjustable flow and shear stress conditions are described in the Methods in the Online Data Supplement. |
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Cells were cultured up to passages 5 through 8 in subtype-specific growth medium (Cell Applications). Cells were seeded on 6-, 12-, or 24-well plates and treated or radiated at a density of 70% to 80%. Endothelial cells were cultured and seeded in gelatin-coated (Attachment Factor Protein 1X, Thermo Fisher Scientific) flasks and plates. |
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Protocol tips |
Cells were cultured up to passages 5 through 8 in subtype-specific growth medium (Cell Applications). Cells were seeded on 6-, 12-, or 24-well plates and treated or radiated at a density of 70% to 80%. Endothelial cells were cultured and seeded in gelatin-coated (Attachment Factor Protein 1X, Thermo Fisher Scientific) flasks and plates. |
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