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Found 3 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
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- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer). |
- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
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Protocol tips |
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer). |
Downstream tips |
- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
|
Upstream tips |
Protocol tips |
Downstream tips |
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1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA
- The chloroform used for phase separation should not contain isoamyl alcohol or other additives.
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Upstream tips |
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1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA
- The chloroform used for phase separation should not contain isoamyl alcohol or other additives.
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Upstream tips |
Protocol tips |
Downstream tips |
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- Keep the Dynabeads™ Oligo (dT)25 in liquid suspension during storage and handling steps.
- All common buffers for mRNA purification and isolation can be used with Dynabeads™ Oligo (dT)25.
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Protocol tips |
- Keep the Dynabeads™ Oligo (dT)25 in liquid suspension during storage and handling steps.
- All common buffers for mRNA purification and isolation can be used with Dynabeads™ Oligo (dT)25.
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