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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| - Cells were pretreated with NAC (10 μM) for 1h. |
- Cells after pretreating with NAC are treated with 5 μM
CM-H2DCFDA or 10 μM DHE for 30 min at 37 °C.
- Tissues were treated with 5 μM CM-H2DCFDA or 10 μM DHE at room temperature for 30 min |
|
| Upstream tips |
| - Cells were pretreated with NAC (10 μM) for 1h. |
| Protocol tips |
- Cells after pretreating with NAC are treated with 5 μM
CM-H2DCFDA or 10 μM DHE for 30 min at 37 °C.
- Tissues were treated with 5 μM CM-H2DCFDA or 10 μM DHE at room temperature for 30 min |
| Upstream tips |
Protocol tips |
Downstream tips |
|
- Cells were treated with Oxidative Stress Detection Reagent after treating them with fixatives (4% paraformaldehyde and 100% acetone).
- Cells were also immunostained with anti-perilipin antibody and TRITC-conjugated anti-rabbit IgG antibody. |
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| Protocol tips |
- Cells were treated with Oxidative Stress Detection Reagent after treating them with fixatives (4% paraformaldehyde and 100% acetone).
- Cells were also immunostained with anti-perilipin antibody and TRITC-conjugated anti-rabbit IgG antibody. |
| Upstream tips |
Protocol tips |
Downstream tips |
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- Differentiated 3T3-L1 cells were cultured for another 2 days and assayed using ROS kit. |
|
| Protocol tips |
| - Differentiated 3T3-L1 cells were cultured for another 2 days and assayed using ROS kit. |
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