ROS assay cell type - A549 human adenocarcinomic human alveolar basal epithelial cells

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 2 matching solutions for this experiment

ROS-ID® Total ROS/Superoxide detection kit

Enzo Life Sciences

Upstream tips	Protocol tips	Downstream tips
- 100,000 cells per well were seeded in a 12 well plate	- Cells were treated with 500 μl of ROS/Superoxide Detection Mix for 30 minutes at 37°C

Upstream tips
- 100,000 cells per well were seeded in a 12 well plate
Protocol tips
- Cells were treated with 500 μl of ROS/Superoxide Detection Mix for 30 minutes at 37°C

Manufacturer protocol Publication protocol 1 Relevant paper

MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
- Cells were pretreated with LGH00168 for 1 h.	- After washing step, cells were incubated with 5 μmol/L of MitoSOX for 20 min in the dark.

Upstream tips
- Cells were pretreated with LGH00168 for 1 h.
Protocol tips
- After washing step, cells were incubated with 5 μmol/L of MitoSOX for 20 min in the dark.

Manufacturer protocol Publication protocol 1 Relevant paper

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