No discussions found
Found 1 matching solution for this experiment
| Prior to differentiation using STEMdiff™ Definitive Endoderm, maintain the cells in their pluripotent state by culturing mTeSR™1 on Matrigel.
plates should be prepared in advance and be brought to room temperature (15 - 25°C) for at least 30 minutes prior to use.
|Differentiate the cells for 5 days using STEMdiff™ Definitive Endoderm media.
-For optimal differentiation performance, cells must be exposed to complete TeSR™-E8™ Pre-Differentiation Medium for at least 24 hours before the next passaging step.