Stem cell Differentiation media Differentiation of Human hESCs into Endoderm

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Upstream tips
Prior to differentiation using STEMdiff™ Definitive Endoderm, maintain the cells in their pluripotent state by culturing mTeSR™1 on Matrigel.
-Matrix-coated
plates should be prepared in advance and be brought to room temperature (15 - 25°C) for at least 30 minutes prior to use.
Protocol tips
Differentiate the cells for 5 days using STEMdiff™ Definitive Endoderm media.
-For optimal differentiation performance, cells must be exposed to complete TeSR™-E8™ Pre-Differentiation Medium for at least 24 hours before the next passaging step.
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