ChIP H3K27me3 Human Donkey

Get tips on using TruSeq ChIP Library Preparation Kit to perform ChIP Human - HUVEC

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Human - HeLa

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - SMMC-7721

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Human - THP-1

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - HEK 293

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - SH-SY5Y

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - MIA PaCa-2

Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Human - Fibroblast cell lines

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.