CytoSelect™ 24-Well Cell Migration Assay, 8 μm

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate for 24 hours in a cell culture incubator	Read fluorescence with a fluorescence plate reader at 480 nm/520 nm.

Protocol tips
Incubate for 24 hours in a cell culture incubator
Downstream tips
Read fluorescence with a fluorescence plate reader at 480 nm/520 nm.

Publication protocol

A CytoSelect™ cell migration assay kit (Cell Biolabs, San Diego, CA, USA) was used for cell migration experiments. Briefly, HUVECs (1x105 ) were seeded into an inner chamber containing 300 µl of L-15 culture medium and placed on a well (outer chamber) of a 24-well plate containing 500 µl of the medium. The cells were incubated for 48-72 h until a monolayer formed. Separately, a total of 5x104 cells/ml of MDA-MB-231 tumor cells were suspended in a serum-free medium and labeled with a CytoTracker™ solution for 1 h. Then, 3x104 of the labeled cells were plated on each inner chamber whose mediu, had been replaced with 300 µl of L-15 medium containing 2 µM SRT1720 and/or 1 µg/ml of cisplatin. These cells were further incubated for 24 h. Non-migrated cells in the inner chamber were removed with cotton-tipped swabs. The cells
that migrated outside the inner chamber were collected and resolved in a lysis buffer. The cell lysate was quantified by measuring at 495 nm (excitation)/520 nm (fluorescence).

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Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Cell Migration Assay, 8 μm below.

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