QCM™ Gelatin Invadopodia Assay (Green)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Place chamber slides into a 5% CO2 tissue culture incubator at 37°C for 20 hours	Increase concentration of blocking serum or protein in fluorescent staining buffer to decrease background if there is a poor staining

Protocol tips
Place chamber slides into a 5% CO2 tissue culture incubator at 37°C for 20 hours
Downstream tips
Increase concentration of blocking serum or protein in fluorescent staining buffer to decrease background if there is a poor staining

Publication protocol

Control and VANGL2 knockdown cells were generated as described.17 The QCMTM gelatin invadopodia assays were performed and quantified according to manufacturer instructions (Millipore, ECM670). In each experiment 20,000 HT-1080 cells were plated per well of an 8-well chamber slide. The total number of degradation spots formed after 20 h incubation (including both invadopodia and other protrusive membrane structures) was quantified from three independent experiments (12 images per experiment = 36 fields of view analyzed, > 500 cells per condition). The number of invadopodia formed after 5 h was also quantified from three independent experiments (20 cells per experiment = 60 total cells per condition). Only cells with at least one invadopodium were analyzed. Cortactin antibody labeling (Millipore clone 4F11; 1:500 dilution) was detected using a mouse Cy5 secondary antibody (Jackson ImmunoResearch; 1:400 dilution). Statistical significance was calculated utilizing the unpaired Student’s ttest.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - HT-1080 using QCM™ Gelatin Invadopodia Assay (Green) from Merck Millipore.

Manufacturer protocol

Download the product protocol from Merck Millipore for QCM™ Gelatin Invadopodia Assay (Green) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell migration / Invasion cell type - HT-1080 using QCM™ Gelatin Invadopodia Assay (Green) from Merck Millipore. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.